便携式生物气溶胶现场采样与初筛系统刑侦与司法鉴定应用介绍

 

便携式生物气溶胶现场采样与初筛系统刑侦与司法鉴定应用介绍

技术原理

1. 高效空气采样与生物样本富集原理

    

   系统内置高性能恒流采样泵，以每分钟 10 升的恒定流速持续抽取环境空气，空气中悬浮的生物气溶胶颗粒物（包括细菌孢子、病毒载体、真菌、生物毒素气溶胶等），通过惯性碰撞与截留效应，被系统内置的 0.8μm 孔径 MCE 微孔滤膜滤芯高效截留，实现空气中微量生物样本的快速富集与干燥保存。该采样过程无化学添加、无生物活性破坏，可完整保留样本中微生物的活性与核酸完整性，采样后的滤芯可直接封存送检，完全满足下游实验室 PCR 扩增、基因测序、微生物培养、法医微生物学鉴定的技术要求，采样流程可全程追溯，符合司法鉴定检材采集的法定规范。
    
2. 现场快速免疫层析检测原理

    

   针对现场可直接获取的固体可疑粉末、液体、生物絮状物等样本，经专用无菌稀释液前处理后，滴加至多通道免疫检测试纸条的加样孔中。系统基于抗原 - 抗体特异性结合的侧向流免疫层析原理，样本中的目标生物因子可与试纸条上的特异性抗体发生特异性结合，在短时间内形成肉眼可识别的显色条带，通过条带判读即可实现对多种特定生物威胁因子的现场快速、并行定性筛查。该检测方法无需复杂仪器设备与专业实验室环境，现场即可完成操作与结果判读，为涉生物事件的快速定性、现场风险分级、应急处置方案制定提供第一时间的技术依据。
    

核心功能与司法实战价值

1. 采样 - 初筛双模协同，构建现场到实验室的完整司法技术链条

    

   系统具备 “现场快速免疫初筛” 与 “实验室送检样本规范化制备” 双重核心功能，形成了从现场预警、快速定性到实验室确证、司法鉴定的完整技术闭环。现场初筛模式下，可在 15 分钟内完成 8 种常见生物威胁因子的并行定性检测，快速锁定风险类型、划定污染范围、指导现场人员防护等级设置；采样富集模式下，可高效富集环境空气中的微量生物气溶胶，有效样本浓度＞1500 CFU/mL，远超常规现场 PCR 分析的检测阈值，为后续实验室精准鉴定、法医微生物检验提供高质量、高活性的司法检材，彻底解决了传统生物物证采样中 “现场无法快速判读、实验室检材富集不足” 的行业痛点。
    
2. 高富集效率恒流采样，保障司法检材的规范性与有效性

    

   系统采用高精度恒流采样控制技术，全程以 10 升 / 分钟的恒定流速完成采样，避免流速波动导致的采样效率偏差，采样过程可记录、可追溯，完全符合司法鉴定检材采集的标准化要求。0.8μm 孔径的 MCE 微孔滤膜对细菌、真菌孢子、病毒气溶胶载体的截留效率＞99%，可在 5 分钟内完成 100 APCL 级别枯草芽孢杆菌黑色变种孢子的有效收集，哪怕是环境中微量的生物气溶胶，也能实现高效富集，从根源上保障了检材的完整性与检验成功率。采样后的滤芯可直接无菌封存，完整保留生物样本的活性与核酸完整性，可直接作为司法鉴定物证送往具备资质的刑事技术实验室进行检验鉴定，为出具具备法律效力的司法鉴定意见提供坚实的检材基础。
    
3. 极致便携快速部署，适配应急处置与现场勘查实战需求

    

   设备采用高度一体化紧凑设计，主机尺寸仅约 8.9cm × 6.4cm × 17.1cm，主机重量仅约 0.8kg，可单手持握、便携收纳，无需复杂安装调试，开箱即可投入使用。机身集成磁性吸附装置，可快速、稳固地附着于车辆、门窗、管道、设备外壳等金属表面，实现数秒内完成现场布设与回收，完美适配突发应急事件的快速响应需求。可选配 12V DC 长续航电池模块，在无市电的野外、偏远现场、封闭污染区域，可实现连续 12 小时以上的不间断采样监测，满足长时间现场布控、大范围环境筛查、应急持续监测的实战需求。
    
4. 高可靠性宽场景适配，满足复杂环境下的作业要求

    

   系统内舱经过标准生物气溶胶挑战测试验证，采样效率、截留效果、样本保存能力均通过专业机构验证，确保采样结果的可靠性与重复性。整机采用工业级防护设计，具备优异的防尘、防水、抗冲击性能，宽泛的电源适应性可支持市电、车载电源、外置电池多种供电模式，能够完美适配室内封闭现场、野外复杂环境、高温高湿、低温沙尘等多种极端场景的采样作业，无论是涉生物案件的室内现场勘查，还是野外边境区域的生物安全监测，均可稳定可靠运行。
    
5. 标准化勤务化全套装配置，保障应急响应的及时性与操作规范性

    

   系统提供标准勤务化完整套装，包含采样主机、备用无菌滤芯、多通道免疫检测试纸条、专用无菌稀释液、无菌采样工具、样本封存袋、便携防护箱等全套配件，开箱即可投入实战部署，无需额外准备耗材，大幅缩短应急响应准备时间。所有耗材均采用无菌独立包装，符合生物安全操作规范与司法鉴定检材采集要求，可有效避免采样过程中的交叉污染，保障检材的纯净性与合法性，同时也降低了一线操作人员的生物安全风险。
    

刑事侦查、反恐应急与司法鉴定实战应用场景

1. 生物恐怖袭击与突发公共卫生事件应急处置

    

   是应对生物恐怖袭击、可疑生物制剂投放、匿名可疑粉末信件、突发不明原因生物污染事件的核心应急装备。接到涉生物威胁警情后，可第一时间携带设备抵达现场，快速布设完成现场空气采样与环境筛查，对现场空气、可疑粉末、物体表面残留物进行快速采样与现场初筛，15 分钟内完成常见生物威胁因子的定性检测，为事件性质判定、污染范围划定、现场人员防护等级设置、应急处置方案制定提供第一时间的技术依据，同时规范采集留存样本，为后续实验室确证、事件溯源、案件定性提供合法有效的司法检材。
    
2. 涉生物违法犯罪案件现场勘查与生物物证固定

    

   广泛适用于非法制售、储运、投放危险生物物质、生物投毒、非法种植制毒原植物、非法开展基因编辑等涉生物违法犯罪案件的现场勘查工作。可对涉案生产窝点、储存场所、作案工具、投放现场进行环境空气采样，高效富集收集空气中残留的生物气溶胶物证，固定涉案生物物质的环境残留证据；同时可对现场查获的可疑生物制剂、粉末、培养液等进行现场快速初筛，快速判定物质种类，为案件侦查方向划定、作案事实认定提供关键线索，规范采集的样本可直接用于后续司法鉴定，为案件的定罪量刑提供核心科学证据。
    
3. 重大活动安保与重点场所生物安全常态化监测

    

   适用于重大国际会议、体育赛事、节庆活动等重大活动安保，以及党政机关、口岸、交通枢纽、核生化重点场所的生物安全常态化监测。可在活动场馆、核心区域、通风系统、人员密集通道等关键位置，进行隐蔽式、周期性布设，开展常态化空气采样与生物气溶胶监测，及时发现异常生物气溶胶释放行为，防范生物恐怖袭击、生物安全风险事件的发生，筑牢重大活动与重点场所的生物安全防线。
    
4. 司法鉴定生物检材规范化前端采集与实验室确证衔接

    

   是司法鉴定机构、公安刑事技术实验室开展法医微生物检验、生物物证鉴定的核心前端采样工具。针对涉生物案件的司法鉴定委托，可通过系统完成涉案现场、涉案物品的规范化空气采样与生物样本富集，采样流程全程可追溯、采样参数标准化、样本保存符合检验要求，采集的滤芯样本可直接送往实验室进行法医微生物学、毒理学、基因组学鉴定，确保前端采样与后端实验室检验的无缝衔接，保障鉴定过程的规范性与鉴定意见的法律效力，解决了传统生物检材采样不规范、样本活性不足、检验成功率低的行业难题。
    
5. 边境与野外偏远区域生物安全监测与应急采样

    

   可用于边境线、口岸、偏远山区、林区等区域的非法生物制剂走私、外来入侵物种、动植物疫情的现场监测与应急采样。设备便携易部署、支持长续航电池供电，可在无市电的野外偏远区域完成长时间连续采样，对边境沿线、口岸通道的环境空气进行常态化监测，及时发现非法跨境生物制剂走私、外来生物入侵风险，同时可对突发动植物疫情、不明原因生物污染事件进行现场快速筛查与样本采集，为边境生物安全防控、疫情应急处置提供技术支撑。
    

核心技术参数

• 物理特性：主机尺寸约 8.9cm × 6.4cm × 17.1cm，主机重量约 0.8kg，采用工业级防护设计，机身集成磁性吸附安装结构，可快速完成现场布设。
• 采样性能：恒定气流速率为 10 升 / 分钟，过滤装置采用 0.8μm 孔径 MCE 微孔滤膜，可在 5 分钟内完成 100 APCL 级别枯草芽孢杆菌黑色变种孢子的有效收集，样本富集后有效样本浓度＞1500 CFU/mL，远超常规 PCR 分析检测阈值。
• 电源与续航：主电源为 120V AC，可选配 12V DC 电池模块扩展续航，电池供电模式下连续工作时间≥12 小时，满足无市电环境的长时间作业需求。
• 现场检测能力：采用多通道侧向流免疫层析检测模式，支持 8 种生物威胁因子的并行快速检测，可直接处理固体粉末、液体、生物絮状物等多种类型样本，检测结果通过肉眼可视化显色条带完成判读，无需复杂辅助设备。
• 环境适应性：支持室内、野外多种复杂环境作业，适配市电、车载电源、外置电池多种供电模式，整机具备优异的防尘、防水、抗冲击性能，可在高低温、高湿、沙尘等极端环境下稳定运行。

实战应用案例

1. 某市公安局反恐支队接报，市政府收到一封匿名可疑信件，信件内装有白色粉末，附带威胁话语，疑似生物恐怖袭击事件。反恐支队联合刑事技术、公共卫生应急部门第一时间抵达现场，启动生物安全应急响应。现场处置人员使用本便携式生物气溶胶现场采样与初筛系统，在信件拆封区域、办公区布设多台设备，完成现场环境空气的生物气溶胶采样，同时对信件内的白色粉末进行现场快速免疫初筛。15 分钟内即完成 8 种常见生物威胁因子的并行检测，初步排除高致病性生物因子风险，同时通过系统采集的空气样本，送往刑事技术实验室进行二次确证，最终确认白色粉末为普通面粉，排除生物恐怖袭击风险。整个处置过程中，系统实现了现场快速初筛与实验室确证的无缝衔接，既快速平息了恐慌，也规范留存了完整的司法检材，确保了处置过程的科学性与合法性。
    
2. 某省公安厅治安总队联合农业农村部门，侦办一起非法制售、储运动物疫病病原生物制剂的重大案件。犯罪团伙在偏远乡村的隐蔽窝点内，非法生产、销售高致病性动物疫病活体制剂，严重危害生物安全与畜牧生产安全。专案组在收网行动中，使用本系统对涉案生产窝点、储存仓库、生产设备、运输车辆进行全面的现场勘查，通过系统对窝点内不同区域的环境空气进行采样，富集收集空气中残留的病原生物气溶胶，固定涉案生物制剂的环境残留证据；同时对现场查获的可疑培养液、冻干制剂进行现场快速初筛，快速锁定涉案生物制剂的种类，为案件定性提供了关键线索。系统采集的滤芯样本送往刑事技术实验室进行基因测序与微生物培养，最终出具了具备法律效力的司法鉴定意见，与现场初筛结果形成完整证据链，为案件的定罪量刑提供了核心科学证据，成功捣毁了该非法制售窝点，抓获全部涉案人员 11 名。
    

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Application Introduction of the Portable Bioaerosol On-Site Sampling and Preliminary Screening System in Criminal Investigation and Forensic Identification

Technical Principle

1. Principle of High-Efficiency Air Sampling and Biological Sample Enrichment

    

   The system has a built-in high-performance constant flow sampling pump, which continuously pumps ambient air at a constant flow rate of 10 liters per minute. Bioaerosol particles suspended in the air (including bacterial spores, viral vectors, fungi, biotoxin aerosols, etc.) are efficiently trapped on the built-in MCE microporous membrane filter element with a pore size of 0.8μm through inertial collision and interception effect, realizing rapid enrichment and dry preservation of trace biological samples in the air. This sampling process has no chemical addition and no biological activity damage, and can completely retain the activity and nucleic acid integrity of microorganisms in the sample. The filter element after sampling can be directly sealed and sent for inspection, which fully meets the technical requirements of downstream laboratory PCR amplification, gene sequencing, microbial culture and forensic microbiological identification. The sampling process can be traced throughout, fully complying with the statutory specifications for forensic specimen collection.
    
2. Principle of On-Site Rapid Immunochromatographic Detection

    

   For solid suspicious powder, liquid, biological floc and other samples that can be directly obtained on site, after pretreatment with special sterile diluent, the sample is dropped into the sample well of the multi-channel immunoassay test strip. Based on the lateral flow immunochromatographic principle of antigen-antibody specific binding reaction, the target biological factors in the sample can specifically bind to the specific antibody on the test strip, forming a color-developing band recognizable by the naked eye in a short time. The on-site rapid and parallel qualitative screening of a variety of specific biological threat factors can be realized through the interpretation of the bands. This detection method does not need complex equipment and professional laboratory environment, and the operation and result interpretation can be completed on site, providing the first-time technical basis for the rapid characterization of biological related events, on-site risk classification, and formulation of emergency disposal plans.
    

Core Functions and Forensic Practical Value

1. Dual-Mode Coordination of Sampling and Preliminary Screening, Building a Complete Forensic Technical Chain from Scene to Laboratory

    

   The system has dual core functions of "on-site rapid immunoassay screening" and "standardized preparation of laboratory inspection samples", forming a complete technical closed loop from on-site early warning, rapid characterization to laboratory confirmation and forensic identification. In the on-site preliminary screening mode, the parallel qualitative detection of 8 common biological threat factors can be completed within 15 minutes, which can quickly lock the risk type, delimit the pollution scope, and guide the setting of on-site personnel protection level. In the sampling enrichment mode, it can efficiently enrich the trace bioaerosol in the ambient air, with an effective sample concentration > 1500 CFU/mL, which far exceeds the detection threshold of conventional on-site PCR analysis. It provides high-quality and high-activity forensic specimens for subsequent laboratory accurate identification and forensic microbiological inspection, completely solving the industry pain points of "unable to quickly interpret on site and insufficient enrichment of laboratory specimens" in traditional biological evidence sampling.
    
2. High Enrichment Efficiency Constant Flow Sampling, Ensuring the Standardization and Validity of Forensic Specimens

    

   The system adopts high-precision constant flow sampling control technology, and completes sampling at a constant flow rate of 10 L/min throughout the process, avoiding sampling efficiency deviation caused by flow rate fluctuation. The sampling process is recordable and traceable, fully complying with the standardized requirements of forensic specimen collection. The 0.8μm pore size MCE microporous membrane filter has a retention efficiency of >99% for bacteria, fungal spores and viral aerosol carriers, and can effectively collect 100 APCL level Bacillus atrophaeus spores within 5 minutes. Even trace bioaerosols in the environment can be efficiently enriched, fundamentally ensuring the integrity of the specimen and the success rate of inspection. The filter element after sampling can be directly sealed aseptically, which completely retains the activity and nucleic acid integrity of the biological sample. It can be directly sent to the qualified criminal technology laboratory for inspection and identification as forensic evidence, providing a solid specimen basis for the issuance of forensic opinions with legal effect.
    
3. Extremely Portable and Rapid Deployment, Adapting to the Practical Needs of Emergency Disposal and Scene Investigation

    

   The equipment adopts a highly integrated and compact design, with the host size of only about 8.9cm × 6.4cm × 17.1cm, and the host weight of only about 0.8kg. It can be held in one hand and stored portably, without complex installation and debugging, and can be put into actual combat deployment out of the box. The fuselage is integrated with a magnetic adsorption device, which can be quickly and stably attached to metal surfaces such as vehicles, doors and windows, pipelines, equipment shells, realizing on-site deployment and recovery within a few seconds, which perfectly adapts to the rapid response needs of emergency events. It can be equipped with an optional 12V DC long-life battery module, which can realize continuous sampling and monitoring for more than 12 hours in the field, remote scenes, closed polluted areas without mains power, meeting the practical needs of long-time on-site deployment, large-scale environmental screening and emergency continuous monitoring.
    
4. High Reliability and Wide Scene Adaptation, Meeting the Operation Requirements in Complex Environments

    

   The inner chamber of the system has been verified by standard bioaerosol challenge tests, and the sampling efficiency, interception effect and sample preservation capacity have been verified by professional institutions to ensure the reliability and repeatability of sampling results. The whole machine adopts industrial-grade protective design, with excellent dustproof, waterproof and impact resistance performance. The wide power supply adaptability can support multiple power supply modes such as mains power, vehicle power supply and external battery. It can perfectly adapt to the sampling operation in various extreme scenes such as indoor closed scenes, complex field environments, high temperature and high humidity, low temperature and sand dust. It can operate stably and reliably whether it is indoor scene investigation of biological related cases or biosafety monitoring in border areas in the wild.
    
5. Standardized Service Full Set Configuration, Ensuring the Timeliness of Emergency Response and Standardization of Operation

    

   The system provides a complete set of standard service configuration, including sampling host, spare sterile filter elements, multi-channel immunoassay test strips, special sterile diluent, sterile sampling tools, sample sealing bags, portable protective box and other full set of accessories. It can be put into actual combat deployment out of the box, no additional consumables are needed, which greatly shortens the preparation time for emergency response. All consumables are packaged in sterile independent packages, which comply with biosafety operation specifications and forensic specimen collection requirements, which can effectively avoid cross-contamination during sampling, ensure the purity and legality of specimens, and also reduce the biosafety risk of front-line operators.
    

Practical Application Scenarios in Criminal Investigation, Anti-Terrorism Emergency and Forensic Identification

1. Emergency Disposal of Bioterrorism Attacks and Public Health Emergencies

    

   It is the core emergency equipment for responding to bioterrorism attacks, suspicious biological agent release, anonymous suspicious powder letters, and sudden unexplained biological pollution events. After receiving the biological threat alarm, the personnel can arrive at the scene with the equipment at the first time, quickly deploy to complete on-site air sampling and environmental screening, conduct rapid sampling and on-site preliminary screening of on-site air, suspicious powder, and object surface residues, and complete the qualitative detection of common biological threat factors within 15 minutes. It provides the first-time technical basis for the determination of the nature of the event, the delimitation of the pollution scope, the setting of on-site personnel protection level, and the formulation of emergency disposal plans. At the same time, the collected samples are standardized and retained, providing legal and effective forensic specimens for subsequent laboratory confirmation, event traceability and case characterization.
    
2. On-Site Investigation and Biological Evidence Fixation of Criminal Cases Involving Biology

    

   It is widely used in the on-site investigation of criminal cases involving biology, such as illegal production, sale, storage and transportation of dangerous biological substances, biological poisoning, illegal planting of drug-producing plants, and illegal gene editing. It can conduct ambient air sampling on the involved production dens, storage places, crime tools, and delivery sites, efficiently enrich and collect the residual bioaerosol evidence in the air, and fix the environmental residual evidence of the involved biological substances. At the same time, it can conduct on-site rapid preliminary screening of suspicious biological preparations, powders, culture fluids seized at the scene, quickly determine the type of substances, provide key clues for the delineation of case investigation direction and the determination of crime facts. The standardized collected samples can be directly used for subsequent forensic identification, providing core scientific evidence for the conviction and sentencing of the case.
    
3. Biosafety Monitoring of Major Event Security and Key Places

    

   It is suitable for the security of major events such as major international conferences, sports events, festivals, as well as the normalized biosafety monitoring of party and government organs, ports, transportation hubs, and nuclear and biochemical key places. It can be deployed covertly and periodically in key positions such as event venues, core areas, ventilation systems, and crowded passages to carry out normalized air sampling and bioaerosol monitoring, timely detect abnormal bioaerosol release behavior, prevent bioterrorism attacks and biosafety risk events, and build a biosafety defense line for major events and key places.
    
4. Standardized Front-End Collection of Forensic Biological Specimens and Connection with Laboratory Confirmation

    

   It is the core front-end sampling tool for forensic institutions and public security criminal technology laboratories to carry out forensic microbiological inspection and biological evidence identification. For the forensic entrustment of biological related cases, the system can be used to complete standardized air sampling and biological sample enrichment of the involved scene and involved items. The sampling process is traceable throughout, the sampling parameters are standardized, and the sample preservation meets the inspection requirements. The collected filter element samples can be directly sent to the laboratory for forensic microbiological, toxicological and genomic identification, ensuring the seamless connection between front-end sampling and back-end laboratory inspection, ensuring the standardization of the identification process and the legal effect of the identification opinions, and solving the industry pain points of non-standard sampling, insufficient sample activity and low inspection success rate of traditional biological specimens.
    
5. Biosafety Monitoring and Emergency Sampling in Border and Remote Field Areas

    

   It can be used for on-site monitoring and emergency sampling of illegal smuggling of biological preparations, invasive alien species, animal and plant epidemics in border lines, ports, remote mountainous areas, forest areas and other areas. The equipment is portable and easy to deploy, supports long-life battery power supply, and can complete long-time continuous sampling in remote field areas without mains power. It can carry out normalized monitoring of ambient air along the border and port channels, timely detect the risk of illegal cross-border smuggling of biological preparations and invasion of alien organisms. At the same time, it can conduct on-site rapid screening and sample collection of sudden animal and plant epidemics and unexplained biological pollution events, providing technical support for border biosafety prevention and control and epidemic emergency disposal.
    

Core Technical Parameters

• Physical Characteristics: The host size is about 8.9cm × 6.4cm × 17.1cm, with a host weight of about 0.8kg. It adopts industrial-grade protective design, and the fuselage is integrated with a magnetic adsorption installation structure, which can quickly complete on-site deployment.
• Sampling Performance: The constant air flow rate is 10 L/min, the filter device adopts 0.8μm pore size MCE microporous membrane filter, which can effectively collect 100 APCL level Bacillus atrophaeus spores within 5 minutes. The effective sample concentration after enrichment is > 1500 CFU/mL, which far exceeds the detection threshold of conventional PCR analysis.
• Power Supply and Battery Life: The main power supply is 120V AC, and it can be optionally equipped with a 12V DC battery module to expand battery life. The continuous working time is ≥12 hours in battery power supply mode, which meets the needs of long-time operation in the environment without mains power.
• On-Site Detection Capability: It adopts multi-channel lateral flow immunochromatographic detection mode, supports parallel rapid detection of 8 biological threat factors, can directly process various types of samples such as solid powder, liquid, and biological floc. The test results are interpreted through naked eye visual color bands without complex auxiliary equipment.
• Environmental Adaptability: It supports operation in various complex environments such as indoor and field, adapts to multiple power supply modes such as mains power, vehicle power supply and external battery. The whole machine has excellent dustproof, waterproof and impact resistance performance, and can operate stably in extreme environments such as high and low temperature, high humidity and sand dust.

Practical Application Cases

1. The Anti-Terrorism Detachment of the Municipal Public Security Bureau received a report that the municipal government received an anonymous suspicious letter with white powder inside and threatening words, which was suspected to be a bioterrorism attack. The anti-terrorism detachment, together with the criminal technology and public health emergency departments, arrived at the scene at the first time and launched the biosafety emergency response. The on-site disposal personnel used this Portable Bioaerosol On-Site Sampling and Preliminary Screening System, deployed multiple sets of equipment in the letter opening area and office area, completed the bioaerosol sampling of the on-site ambient air, and conducted on-site rapid immunoassay preliminary screening of the white powder in the letter. Within 15 minutes, the parallel detection of 8 common biological threat factors was completed, and the risk of highly pathogenic biological factors was initially ruled out. At the same time, the air samples collected by the system were sent to the criminal technology laboratory for secondary confirmation, and finally it was confirmed that the white powder was ordinary flour, and the risk of bioterrorism attack was ruled out. During the whole disposal process, the system realized the seamless connection between on-site rapid preliminary screening and laboratory confirmation, which not only quickly quelled the panic, but also standardized and retained the complete forensic specimens, ensuring the scientificity and legality of the disposal process.
    
2. The Public Security Corps of the Provincial Public Security Department, together with the agricultural and rural departments, investigated and handled a major case of illegal production, sale, storage and transportation of animal epidemic pathogenic biological preparations. The criminal gang illegally produced and sold live preparations of highly pathogenic animal diseases in a hidden den in a remote rural village, which seriously endangered biosafety and animal husbandry production safety. During the arrest operation, the task force used this system to conduct a comprehensive on-site investigation of the involved production den, storage warehouse, production equipment and transport vehicles. Through the system, the ambient air in different areas of the den was sampled, the residual pathogenic bioaerosol in the air was enriched and collected, and the environmental residual evidence of the involved biological preparations was fixed. At the same time, the on-site rapid preliminary screening was carried out on the suspicious culture fluid and freeze-dried preparations seized at the scene, which quickly locked the type of the involved biological preparations and provided key clues for the case characterization. The filter element samples collected by the system were sent to the criminal technology laboratory for gene sequencing and microbial culture, and finally issued a forensic opinion with legal effect, which formed a complete evidence chain with the on-site preliminary screening results. It provided core scientific evidence for the conviction and sentencing of the case, successfully destroyed the illegal production and sale den, and arrested all 11 people involved.